Afrifix Oil Spill Bioremediation


Cutting edge proprietary developed environmentally friendly enzyme producing bacteria hydrocarbon soil remediating solution for all oil, diesel, petrol, fuel and hydrocarbon associated spills. Bioremediation refers to any method that uses microbes to recycle organic materials and sequester inorganic ions. Under carefully controlled conditions, bioremediation can be a practical and cost effective method to remove hydrocarbons and other organics from contaminated surfaces.

Recommended Applications:
In order to have hyper-degradation occur there must be a minimum 1 x 107 oil degrading microbes per gram of contaminant to override the indigenous population of microbes. The only lab test required is a simple, quick, and inexpensive biocompatibility test to determine if there are any substances present that would be toxic to the microbes, thus preventing them from doing their job. Once a site is inoculated, the microbes quickly adapt to their new environment and begin the break-down of the pollutant. The speed with which this occurs is relative to the density of the needed microbe population. The microbial product is a consortium of billions hydrocarbon digesting microbes in various carriers, and since growth of the microbes is exponential; the pollutant is quickly and effciently degraded, resulting in a faster treatment or closure. As in all products, water and oxygen must be present to allow the microbes to break down organic waste into carbon dioxide, water, and soluble fatty acids. When activated by water, they provide our customers the opportunity to activate the microbes at their convenience. Because microbes are living assemblage, it must be understood that they can be destroyed by caustics, high concentrations of chlorine, some solvents, and chemicals. X-raying the microbes will kill and destroy them. Other conditions must be taken into account, such as ph, temperature, salinity, type of contaminant, etc.

Directions For Use:
1. Firstly, if it is a fresh spill, it should first be contained using high efficiency eco-friendly absorbents. These absorbents are poured around the spill to make sure it does not spread. It also absorbs the excess oil.
2. The soil is then dug up to the deepest level of contamination, and the total affected cubic volume is calculated. Take Total Petroleum Hydrocarbon (T.P.H.) readings to ascertain the level of soil contamination before treatment begins as well as pH readings. The pH must be in the 5,5 to 8,5 region.
3. The soil is either treated in place (in situ) or moved into a bunded containment area (ex situ).
4. If any large clods of soil exist, they should first be broken up into smaller pieces to ensure even coverage by the microbes.
5. The microbes and activation nutrients present in the powder are then mixed into the soil. 1kg of powder is used for 1m3 of soil to be treated per dose. In some less severe cases, a double dose may be used where it is suspected likely to complete the treatment in a single treatment step.
6. To ensure a more even dispersion of microbes, the powder can first be mixed into a carrier/adsorbent such as coco-peat, coco-husk or clay. In this case, the microbes are first mixed into the carrier/adsorbent and then the entire contents are mixed into the soil. Mix at a ratio of 1:10, i.e. 1Kg Oil Spill Bioremediator Powder plus 9Kg carrier/adsorbent.
7. The soil moisture content should ideally be in the 20% to 30% range. If the soil moisture content is below that level, then the soil should be sprayed with water to achieve the ideal moisture content. When the level of contamination is severe or a quicker result is required, Afrifix’s bio-degradable bacteria and environmentally friendly degreaser, Marine Oil Spill Bioremediator, is added and sprayed to assist in the remedial process. This has the effect of significantly increasing surface area available, and consequently assisting the microbes bind with and penetrate the oil interface more effectively. Please see Marine Oil Spill Bioremediator for more information.
8. In some cases Afrifix Oil Spill Bioremediation water and Marine Oil Spill Bioremediator may be premixed and sprayed onto the soil passing on a conveyor belt system to enhance emulsification and provide even dispersion.
9. Depending on the scale of the work, the treatment may need to be mechanised as per point 8 above.
10. The treatment procedure is typically repeated once monthly. Depending on the nature and load of T.P.H. the complete treatment process can take anywhere between 1 to 9 months. Soil samples are taken and measured for T.P.H. to determine the level of treatment, which informs whether another dose is necessary.
11. The more microbes and nutrients are added, the faster and more efficient the degradation process becomes.

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